Journal: PLoS ONE
Article Title: Bivalirudin in Combination with Heparin to Control Mesenchymal Cell Procoagulant Activity
doi: 10.1371/journal.pone.0042819
Figure Lengend Snippet: -hALPCs PCA in ROTEM (Blood). Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of citrated whole blood (300 µl) in presence or not of cells suspended in human albumin 5%. No coagulation was induced in the absence of recalcification. Hepatocytes (white), human adult liver progenitor cells (hALPCs) (black), control (albumin) (grey). * as compared to hALPCs f as compared to control hALPCs vs. hepatocytes vs. control. ***p<0.001 (Kruskal-Wallis test). -hALPCs PCA in ROTEM (Plasma). Clotting time (CT) assayed by ROTEM after recalcification, with added tissue factor (ExTem 20 µL) of plasma (300 µl) obtained from blood incubated in presence or not of cells suspended in human albumin 5%. Hepatocytes (white), human adult liver progenitor cells (hALPCs) (black), control (albumin) (grey). * as compared to hALPCs f as compared to control hALPCs vs. hepatocytes vs. control. ***p<0.001 (Kruskal-Wallis test). -hALPCS PCA in ROTEM (no TF addition). Clotting time (CT) essayed by ROTEM after recalcification, without added Tissue Factor (ExTem 20 µL), of citrated whole blood (300 µl) in presence or not of cells suspended in human albumin 5%. No coagulation is induced if absence of recalcification. Hepatocytes (white), hALPCs (black).
Article Snippet: Measurements were performed on a ROTEM® delta analyser (Pentapharm, Munich, Germany).
Techniques: Coagulation, Incubation